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Fisher Scientific lipofectamine 3000 transfection reagent
Lipofectamine 3000 Transfection Reagent, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine+3000+transfection+reagent/3000+lipofectamine/pm41846530-49-4-8
Average 86 stars, based on 1 article reviews
lipofectamine 3000 transfection reagent - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Transfection:

Article Title: Immunomodulation of behavior impairment via spleen-specific targeting lipid nanoparticles in a MeCP2 transgenic mouse model.
Article Snippet: This cell line was cultured in RPMI-1640 (ATCC, Catalog#30–2001) supplemented with 10% of fetal bovine serum (Fisher Scientific, Catalog#16-000−044). .. Lipofectamine 3000 Transfection Reagent (Fisher Scientific, Catalog#L3000001) was a benchmark control. .. The LNP in vitro cytotoxicity test followed the manufacturer’s instructions for cell counting kit 8 (CCK8, Abcam, catalog#ab228554).

Article Title: Immunomodulation of behavior impairment via spleen-specific targeting lipid nanoparticles in a MeCP2 transgenic mouse model
Article Snippet: This cell line was cultured in RPMI-1640 (ATCC, Catalog#30–2001) supplemented with 10% of fetal bovine serum (Fisher Scientific, Catalog#16-000−044). .. Lipofectamine 3000 Transfection Reagent (Fisher Scientific, Catalog#L3000001) was a benchmark control. .. The LNP in vitro cytotoxicity test followed the manufacturer’s instructions for cell counting kit 8 (CCK8, Abcam, catalog#ab228554).

Article Title: Integrating fragment-based screening with targeted protein degradation and genetic rescue to explore eIF4E function.
Article Snippet: .. Plasmids (2.5μg) were transfected into cells using Lipofectamine 3000 transfection reagent (Fisher Scientific, catL3000008) in OptiMem (Fisher Scientific, cat#11058021) and left for 6 hr before adding fresh media for 48 hr. .. Depending on the selection encoded by the plasmid construct transfected cells were selected using 1μg/ml puromycin (Fisher Scientific, cat#A1113802) or 400μg/ml hygromycin (Fisher Scientific, cat#10687010).

Article Title: Systematic decoding of cis gene regulation defines context-dependent control of the multi-gene costimulatory receptor locus in human T cells
Article Snippet: Lenti-X cells were plated overnight in complete Opti-MEM (Gibco, 31985088; supplemented with 5% FCS (R&D Systems, lot M19187), 1 mM sodium pyruvate and 1× MEM non-essential amino acids) to achieve 85–95% confluency the following morning. .. Next, Lenti-X cells were transfected with the desired plasmid, the second-generation lentiviral packaging plasmid psPAX2 and the transfer plasmid pMD2.G using Lipofectamine 3000 Transfection Reagent (Fisher Scientific, L3000075). .. After 6 h, the transfection medium was replaced with complete Opti-MEM supplemented with 1.15× ViralBoost (Alstem Bio, VB100).

Article Title: Deregulated miR-487b-3p in Patients with Non-Alcoholic Fatty Liver Disease and Its Regulatory Effect on Insulin Resistance.
Article Snippet: .. Cells were transfected with Lipofectamine 3000 transfection reagent (Fisher Scientific, USA) following the supplier’s technical specifications. ..

Article Title: Systematic decoding of cis gene regulation defines context-dependent control of the multi-gene costimulatory receptor locus in human T cells.
Article Snippet: Lenti-X cells were plated overnight in complete Opti-MEM (Gibco, 31985088; supplemented with 5% FCS (R&D Systems, lot M19187), 1 mM sodium pyruvate and 1× MEM non-essential amino acids) to achieve 85–95% confluency the following morning. .. Next, Lenti-X cells were transfected with the desired plasmid, the second-generation lentiviral packaging plasmid psPAX2 and the transfer plasmid pMD2.G using Lipofectamine 3000 Transfection Reagent (Fisher Scientific, L3000075). .. After 6 h, the transfection medium was replaced with complete Opti-MEM supplemented with 1.15× ViralBoost (Alstem Bio, VB100).

Article Title: Integrating fragment-based screening with targeted protein degradation and genetic rescue to explore eIF4E function.
Article Snippet: .. Proteome Profiling Proteomes were profiled by LC-MS/MS using label-free data-independent acquisition (DIA; BGI address) following 72 hr exposure of the four H1299 eIF4E-dTAG clones (C2-2, C3-1, N2-1 and N3-2) to 0.5μM dTAGV-1 or H1299 parent cells transfected with 1μg eIF4E siRNA (siTOOLS, Planegg, Germany; cat# si-G100-1977) using Lipofectamine 3000 transfection reagent (Fisher Scientific, catL3000008). ..

Control:

Article Title: Immunomodulation of behavior impairment via spleen-specific targeting lipid nanoparticles in a MeCP2 transgenic mouse model.
Article Snippet: This cell line was cultured in RPMI-1640 (ATCC, Catalog#30–2001) supplemented with 10% of fetal bovine serum (Fisher Scientific, Catalog#16-000−044). .. Lipofectamine 3000 Transfection Reagent (Fisher Scientific, Catalog#L3000001) was a benchmark control. .. The LNP in vitro cytotoxicity test followed the manufacturer’s instructions for cell counting kit 8 (CCK8, Abcam, catalog#ab228554).

Article Title: Immunomodulation of behavior impairment via spleen-specific targeting lipid nanoparticles in a MeCP2 transgenic mouse model
Article Snippet: This cell line was cultured in RPMI-1640 (ATCC, Catalog#30–2001) supplemented with 10% of fetal bovine serum (Fisher Scientific, Catalog#16-000−044). .. Lipofectamine 3000 Transfection Reagent (Fisher Scientific, Catalog#L3000001) was a benchmark control. .. The LNP in vitro cytotoxicity test followed the manufacturer’s instructions for cell counting kit 8 (CCK8, Abcam, catalog#ab228554).

other:

Article Title: Pre-mRNA splicing order is predetermined and maintains splicing fidelity across multi-intronic transcripts
Article Snippet: After 24 hours, cells were transfected with 250 pmoles of antisense oligonucleotide (ASO) 22 and 10 uL of Lipofectamine RNAiMAX (Fisher Scientific, 13–778-030) per well according to manufacturer’s instructions for forward transfection.

Plasmid Preparation:

Article Title: Systematic decoding of cis gene regulation defines context-dependent control of the multi-gene costimulatory receptor locus in human T cells
Article Snippet: Lenti-X cells were plated overnight in complete Opti-MEM (Gibco, 31985088; supplemented with 5% FCS (R&D Systems, lot M19187), 1 mM sodium pyruvate and 1× MEM non-essential amino acids) to achieve 85–95% confluency the following morning. .. Next, Lenti-X cells were transfected with the desired plasmid, the second-generation lentiviral packaging plasmid psPAX2 and the transfer plasmid pMD2.G using Lipofectamine 3000 Transfection Reagent (Fisher Scientific, L3000075). .. After 6 h, the transfection medium was replaced with complete Opti-MEM supplemented with 1.15× ViralBoost (Alstem Bio, VB100).

Article Title: Systematic decoding of cis gene regulation defines context-dependent control of the multi-gene costimulatory receptor locus in human T cells.
Article Snippet: Lenti-X cells were plated overnight in complete Opti-MEM (Gibco, 31985088; supplemented with 5% FCS (R&D Systems, lot M19187), 1 mM sodium pyruvate and 1× MEM non-essential amino acids) to achieve 85–95% confluency the following morning. .. Next, Lenti-X cells were transfected with the desired plasmid, the second-generation lentiviral packaging plasmid psPAX2 and the transfer plasmid pMD2.G using Lipofectamine 3000 Transfection Reagent (Fisher Scientific, L3000075). .. After 6 h, the transfection medium was replaced with complete Opti-MEM supplemented with 1.15× ViralBoost (Alstem Bio, VB100).

Data-independent acquisition:

Article Title: Integrating fragment-based screening with targeted protein degradation and genetic rescue to explore eIF4E function.
Article Snippet: .. Proteome Profiling Proteomes were profiled by LC-MS/MS using label-free data-independent acquisition (DIA; BGI address) following 72 hr exposure of the four H1299 eIF4E-dTAG clones (C2-2, C3-1, N2-1 and N3-2) to 0.5μM dTAGV-1 or H1299 parent cells transfected with 1μg eIF4E siRNA (siTOOLS, Planegg, Germany; cat# si-G100-1977) using Lipofectamine 3000 transfection reagent (Fisher Scientific, catL3000008). ..

Clone Assay:

Article Title: Integrating fragment-based screening with targeted protein degradation and genetic rescue to explore eIF4E function.
Article Snippet: .. Proteome Profiling Proteomes were profiled by LC-MS/MS using label-free data-independent acquisition (DIA; BGI address) following 72 hr exposure of the four H1299 eIF4E-dTAG clones (C2-2, C3-1, N2-1 and N3-2) to 0.5μM dTAGV-1 or H1299 parent cells transfected with 1μg eIF4E siRNA (siTOOLS, Planegg, Germany; cat# si-G100-1977) using Lipofectamine 3000 transfection reagent (Fisher Scientific, catL3000008). ..



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